Methods: Cell culture in serum - free medium , indirect immunofluorescence cytochemistry were used.

方法: 采用无血清细胞培养技术, 间接免疫细胞化学染色法.

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Serum free amino acid was measured before and after the treatment.

结果:经碳酸盐透析后,患者血浆大多数游离氨基酸和总氨基酸含量显著降低.

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CONCLUSION: The keratinocytes growed best in serum - free medium supplemented with EGF and BPE.

结论: 细胞在加入表皮生长因子和促进增殖的垂体提取物的无血清培养基中增殖良好.

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Conclusion: The serum - containing medium for culture of CHO cells can be completely substituted by serum - free media.

结论: 无血清培养基可以完全取代含血清培养基用于培养CHO细胞.

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Methods: The technique of serum free cultivation and the methods of immunofluorescence cytochemistry were used.

方法利用无血清培养技术和细胞免疫化学方法.

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