After PCR and sequencing, the expression plasmid pWD κ H was transfected into CHO ( dhfr ~ - ) cells.

经PCR和测序鉴定正确后, 用lipofectAMINE2 000转染CHO ( dhfr - ) 细胞.

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SDS - PAGE showed that the fused protein of interest has been specifically expressed as 61 kD.

所得菌体总蛋白经SDS -PAGE 分析,结果表明,在预期的61kD处 得到一条特异带,为AK( 1-3)cDNA与 载体固有基因DHFR-6хHis的重组蛋白表达产物.

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A novel screening method for dihydrofolate reductase inhibitors ( DHFRI ) using high performance capillary electrophoresis ( HPCE ) was developed.

建立了一种采用毛细管电泳法 ( CE ) 测定二氢叶酸还原酶 ( DHFR ) 反应动力学参数的新方法.

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